Affinity chromatography
The method of separation of biochemical mixture is known as Affinity chromatography. Separation of biochemical mixture based on a is highly specific interaction between
· Antigen and antibody
· Enzyme and substrate
· Receptor and ligand
· Protein and nucleic acid
It consists of two phases a stationary and mobile phase. Some molecules from the mobile phase will bond to the stationary phase based on their properties; the rest of the solution passes away. This process creates a rapid interaction between the desired protein molecules and the stationary molecules by this we can get highly purified product.
Due to the high selectivity; it can be used for purifications. Affinity chromatography helps in diagnosis its key tool for immune chromatographic tests. It’s a unique technique that enables the purification of a biomolecules based upon their biological function including nucleic acid purification, protein purification from cell free extracts, and purification from blood.
Biochemistry and Modern Applications is a peer reviewed Journal, with rapid publication process. The topics like DNA polymerases, Heterochromatin, Ribosome, Non-coding DNA, Cell biology, Metabolism, Nutritional Biochemistry, Medicinal Biochemistry and Hormonal Biochemistry are studied. This is not just limited to above areas. The knowledge related to different biomolecules and their mechanisms can be studied in the Journal of Biochemistry and Modern Applications.
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